goat anti zo1 Search Results


goat anti-zo-1  (Arigo Biolaboratories)
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Arigo Biolaboratories goat anti-zo-1
Fut2 deficiency aggravated the epithelial tight junction and mucus barrier damage in DSS-induced colitis. a The typical immunofluorescent images of colon tissues stained with <t>ZO-1</t> (green), occludin (red), the principal components of tight junction (Scale bar, 100 μm) b Relative mRNA expression of occludin and ZO-1 in different groups. c The typical histological images of colon tissue stained with AB/PAS from different groups (Scale bar, 100 μm). d The number of goblet cells (Scale bar, 50 μm). Data were shown as mean ± SEM; (n = 5 per group). The data come from three independent experiments. In all panels: * p < 0.05, ** p < 0.01, *** p < 0.001
Goat Anti Zo 1, supplied by Arigo Biolaboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
goat anti-zo-1 - by Bioz Stars, 2026-03
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Fut2 deficiency aggravated the epithelial tight junction and mucus barrier damage in DSS-induced colitis. a The typical immunofluorescent images of colon tissues stained with ZO-1 (green), occludin (red), the principal components of tight junction (Scale bar, 100 μm) b Relative mRNA expression of occludin and ZO-1 in different groups. c The typical histological images of colon tissue stained with AB/PAS from different groups (Scale bar, 100 μm). d The number of goblet cells (Scale bar, 50 μm). Data were shown as mean ± SEM; (n = 5 per group). The data come from three independent experiments. In all panels: * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Journal of Biomedical Science

Article Title: Gut microbiota-mediated lysophosphatidylcholine generation promotes colitis in intestinal epithelium-specific Fut2 deficiency

doi: 10.1186/s12929-021-00711-z

Figure Lengend Snippet: Fut2 deficiency aggravated the epithelial tight junction and mucus barrier damage in DSS-induced colitis. a The typical immunofluorescent images of colon tissues stained with ZO-1 (green), occludin (red), the principal components of tight junction (Scale bar, 100 μm) b Relative mRNA expression of occludin and ZO-1 in different groups. c The typical histological images of colon tissue stained with AB/PAS from different groups (Scale bar, 100 μm). d The number of goblet cells (Scale bar, 50 μm). Data were shown as mean ± SEM; (n = 5 per group). The data come from three independent experiments. In all panels: * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Antibodies used were: goat anti-ZO-1 (1:200, arigo) and rabbit anti-occludin (1:200, abcam).

Techniques: Staining, Expressing

Gut microbiota from Fut2 △IEC mice promotes the generation of LPC, aggravating colitis a Body weight change of mice in FMT-WT group and FMT-F group during the process. b DAI evaluations for the two groups. (C,D) The length of colons from each group of mice. e Representative H&E staining images of colonic sections. f Immunofluorescent images of colon tissues stained with ZO-1 (green), and occludin (red) in each group (Scale bar, 100 μm). g The proteins level of TNF-α, IL-1β in colon tissues. GAPDH was used as a loading control. (H) Fecal concentration of LPC of mice in each group. Data are expressed as mean ± SEM (n = 5 per group). The data come from three independent experiments. In all panels: * p < 0.05, ** p < 0.01, *** p < 0.001. ( LPC lysophosphatidylcholine, H&E hematoxylin and eosin)

Journal: Journal of Biomedical Science

Article Title: Gut microbiota-mediated lysophosphatidylcholine generation promotes colitis in intestinal epithelium-specific Fut2 deficiency

doi: 10.1186/s12929-021-00711-z

Figure Lengend Snippet: Gut microbiota from Fut2 △IEC mice promotes the generation of LPC, aggravating colitis a Body weight change of mice in FMT-WT group and FMT-F group during the process. b DAI evaluations for the two groups. (C,D) The length of colons from each group of mice. e Representative H&E staining images of colonic sections. f Immunofluorescent images of colon tissues stained with ZO-1 (green), and occludin (red) in each group (Scale bar, 100 μm). g The proteins level of TNF-α, IL-1β in colon tissues. GAPDH was used as a loading control. (H) Fecal concentration of LPC of mice in each group. Data are expressed as mean ± SEM (n = 5 per group). The data come from three independent experiments. In all panels: * p < 0.05, ** p < 0.01, *** p < 0.001. ( LPC lysophosphatidylcholine, H&E hematoxylin and eosin)

Article Snippet: Antibodies used were: goat anti-ZO-1 (1:200, arigo) and rabbit anti-occludin (1:200, abcam).

Techniques: Staining, Concentration Assay

LPC impaired intestinal barrier function in vitro and in vivo . a Relative mRNA and b , c Protein expression of occludin and ZO-1 in colon tissues. GAPDH was used as a loading control. d The typical immunofluorescent images of colon tissues stained with ZO-1 (green), and occludin (red) in each group (Scale bar, 100 μm). e , f The typical images of colon tissue stained with AB/PAS and the number of goblet cells from two groups (Scale bar, 100 μm). g TEER of Caco-2 cells treated or not treated with LPC. (H) The mRNA level of ZO-1 and occludin in caco-2 cells treated with LPC of two groups. i , j The protein levels of ZO-1 and occludin in caco-2 cells treated with LPC of two groups. k The immunofluorescent images of Caco-2 cells with ZO-1 and occludin in each group (Z-axis and XY-axis). Data were shown as mean ± SEM (n = 6 per group). The data come from three independent experiments. In all panels: * p < 0.05. ( AB/PAS alcian blue/periodic acid Schiff, TEER transepithelial/transendothelial electrical resistance)

Journal: Journal of Biomedical Science

Article Title: Gut microbiota-mediated lysophosphatidylcholine generation promotes colitis in intestinal epithelium-specific Fut2 deficiency

doi: 10.1186/s12929-021-00711-z

Figure Lengend Snippet: LPC impaired intestinal barrier function in vitro and in vivo . a Relative mRNA and b , c Protein expression of occludin and ZO-1 in colon tissues. GAPDH was used as a loading control. d The typical immunofluorescent images of colon tissues stained with ZO-1 (green), and occludin (red) in each group (Scale bar, 100 μm). e , f The typical images of colon tissue stained with AB/PAS and the number of goblet cells from two groups (Scale bar, 100 μm). g TEER of Caco-2 cells treated or not treated with LPC. (H) The mRNA level of ZO-1 and occludin in caco-2 cells treated with LPC of two groups. i , j The protein levels of ZO-1 and occludin in caco-2 cells treated with LPC of two groups. k The immunofluorescent images of Caco-2 cells with ZO-1 and occludin in each group (Z-axis and XY-axis). Data were shown as mean ± SEM (n = 6 per group). The data come from three independent experiments. In all panels: * p < 0.05. ( AB/PAS alcian blue/periodic acid Schiff, TEER transepithelial/transendothelial electrical resistance)

Article Snippet: Antibodies used were: goat anti-ZO-1 (1:200, arigo) and rabbit anti-occludin (1:200, abcam).

Techniques: In Vitro, In Vivo, Expressing, Staining